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elevated plus maze apparatus  (Noldus Information Technology)
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( A ) Open field beam breaks display no differences in locomotion amongst CTRL and cKO animals (CTRL: 3892 ± 271; cKO: 4076 ± 239, n = 22 mice per group, p = 0.61, Unpaired t -test). ( B ) Upf2 cKO mice show reduced time in open arms of <t>elevated</t> <t>plus</t> <t>maze</t> indicating anxiety-like behavior (CTRL: 38.5 ± 3.9; cKO: 23.09 ± 2.6, n = 31 mice per group, p = 0.0036, Unpaired t -test). ( C ) Astrocytic Upf2 loss is associated with reduced marble burying (CTRL: 16.3 ± 0.6, n = 22 mice; cKO: 13.0 ± 0.8, n = 23 mice, p = 0.0003, Mann-Whitney test). ( D ) Contextual fear conditioning paradigm revealed differences in contextual memory on Day 2 between CTRL and cKO animals (CTRL: 68.3 ± 3, n = 29 mice; cKO: 77.6 ± 3, n = 24 mice, p = 0.02, Mann-Whitney test). No differences were detected on Day 1 and Day 3 (Day 1: Baseline vs. UC-CS pairings, CTRL vs. cKO, p > 0.59, Sidak’s multiple comparisons test; Day 3: CTRL vs. cKO, p = 0.69, Unpaired t -test). Assessment of baseline freezing revealed Upf2 cKO mice show significant elevation on Day 2 and Day 3 as compared to CTRL (Day 1: CTRL: 1.8 ± 0.6; cKO: 6.1 ± 2.1, p = 0.43, Mann-Whitney test. Day 2: CTRL: 12.4 ± 0.8; cKO: 14.6 ± 0.7, p = 0.048, Unpaired t -test. Day 3: CTRL: 21.2 ± 3.6; cKO: 34.9 ± 4.5, p = 0.03, Mann-Whitney test. CTRL: n = 29 mice, cKO: n = 24 mice). Data are presented as mean ± S.E.M. * denotes p < 0.05, ** denotes p < 0.01, *** denotes p < 0.005, ns = not significant. This figure illustrates that astrocytic UPF2 depletion is associated with avoidance behavior and increased baseline freezing.
Elevated Plus Maze Apparatus, supplied by Noldus Information Technology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plus maze apparatus  (UGO Basile S.R.L)
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( A ) Open field beam breaks display no differences in locomotion amongst CTRL and cKO animals (CTRL: 3892 ± 271; cKO: 4076 ± 239, n = 22 mice per group, p = 0.61, Unpaired t -test). ( B ) Upf2 cKO mice show reduced time in open arms of <t>elevated</t> <t>plus</t> <t>maze</t> indicating anxiety-like behavior (CTRL: 38.5 ± 3.9; cKO: 23.09 ± 2.6, n = 31 mice per group, p = 0.0036, Unpaired t -test). ( C ) Astrocytic Upf2 loss is associated with reduced marble burying (CTRL: 16.3 ± 0.6, n = 22 mice; cKO: 13.0 ± 0.8, n = 23 mice, p = 0.0003, Mann-Whitney test). ( D ) Contextual fear conditioning paradigm revealed differences in contextual memory on Day 2 between CTRL and cKO animals (CTRL: 68.3 ± 3, n = 29 mice; cKO: 77.6 ± 3, n = 24 mice, p = 0.02, Mann-Whitney test). No differences were detected on Day 1 and Day 3 (Day 1: Baseline vs. UC-CS pairings, CTRL vs. cKO, p > 0.59, Sidak’s multiple comparisons test; Day 3: CTRL vs. cKO, p = 0.69, Unpaired t -test). Assessment of baseline freezing revealed Upf2 cKO mice show significant elevation on Day 2 and Day 3 as compared to CTRL (Day 1: CTRL: 1.8 ± 0.6; cKO: 6.1 ± 2.1, p = 0.43, Mann-Whitney test. Day 2: CTRL: 12.4 ± 0.8; cKO: 14.6 ± 0.7, p = 0.048, Unpaired t -test. Day 3: CTRL: 21.2 ± 3.6; cKO: 34.9 ± 4.5, p = 0.03, Mann-Whitney test. CTRL: n = 29 mice, cKO: n = 24 mice). Data are presented as mean ± S.E.M. * denotes p < 0.05, ** denotes p < 0.01, *** denotes p < 0.005, ns = not significant. This figure illustrates that astrocytic UPF2 depletion is associated with avoidance behavior and increased baseline freezing.
Plus Maze Apparatus, supplied by UGO Basile S.R.L, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plexiglas plus maze apparatus  (Noldus Information Technology)
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Noldus Information Technology plexiglas plus maze apparatus
( A ) Open field beam breaks display no differences in locomotion amongst CTRL and cKO animals (CTRL: 3892 ± 271; cKO: 4076 ± 239, n = 22 mice per group, p = 0.61, Unpaired t -test). ( B ) Upf2 cKO mice show reduced time in open arms of <t>elevated</t> <t>plus</t> <t>maze</t> indicating anxiety-like behavior (CTRL: 38.5 ± 3.9; cKO: 23.09 ± 2.6, n = 31 mice per group, p = 0.0036, Unpaired t -test). ( C ) Astrocytic Upf2 loss is associated with reduced marble burying (CTRL: 16.3 ± 0.6, n = 22 mice; cKO: 13.0 ± 0.8, n = 23 mice, p = 0.0003, Mann-Whitney test). ( D ) Contextual fear conditioning paradigm revealed differences in contextual memory on Day 2 between CTRL and cKO animals (CTRL: 68.3 ± 3, n = 29 mice; cKO: 77.6 ± 3, n = 24 mice, p = 0.02, Mann-Whitney test). No differences were detected on Day 1 and Day 3 (Day 1: Baseline vs. UC-CS pairings, CTRL vs. cKO, p > 0.59, Sidak’s multiple comparisons test; Day 3: CTRL vs. cKO, p = 0.69, Unpaired t -test). Assessment of baseline freezing revealed Upf2 cKO mice show significant elevation on Day 2 and Day 3 as compared to CTRL (Day 1: CTRL: 1.8 ± 0.6; cKO: 6.1 ± 2.1, p = 0.43, Mann-Whitney test. Day 2: CTRL: 12.4 ± 0.8; cKO: 14.6 ± 0.7, p = 0.048, Unpaired t -test. Day 3: CTRL: 21.2 ± 3.6; cKO: 34.9 ± 4.5, p = 0.03, Mann-Whitney test. CTRL: n = 29 mice, cKO: n = 24 mice). Data are presented as mean ± S.E.M. * denotes p < 0.05, ** denotes p < 0.01, *** denotes p < 0.005, ns = not significant. This figure illustrates that astrocytic UPF2 depletion is associated with avoidance behavior and increased baseline freezing.
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nir backlit epm apparatus catalog env 564a medassociates  (Med Associates Inc)
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( A ) Open field beam breaks display no differences in locomotion amongst CTRL and cKO animals (CTRL: 3892 ± 271; cKO: 4076 ± 239, n = 22 mice per group, p = 0.61, Unpaired t -test). ( B ) Upf2 cKO mice show reduced time in open arms of <t>elevated</t> <t>plus</t> <t>maze</t> indicating anxiety-like behavior (CTRL: 38.5 ± 3.9; cKO: 23.09 ± 2.6, n = 31 mice per group, p = 0.0036, Unpaired t -test). ( C ) Astrocytic Upf2 loss is associated with reduced marble burying (CTRL: 16.3 ± 0.6, n = 22 mice; cKO: 13.0 ± 0.8, n = 23 mice, p = 0.0003, Mann-Whitney test). ( D ) Contextual fear conditioning paradigm revealed differences in contextual memory on Day 2 between CTRL and cKO animals (CTRL: 68.3 ± 3, n = 29 mice; cKO: 77.6 ± 3, n = 24 mice, p = 0.02, Mann-Whitney test). No differences were detected on Day 1 and Day 3 (Day 1: Baseline vs. UC-CS pairings, CTRL vs. cKO, p > 0.59, Sidak’s multiple comparisons test; Day 3: CTRL vs. cKO, p = 0.69, Unpaired t -test). Assessment of baseline freezing revealed Upf2 cKO mice show significant elevation on Day 2 and Day 3 as compared to CTRL (Day 1: CTRL: 1.8 ± 0.6; cKO: 6.1 ± 2.1, p = 0.43, Mann-Whitney test. Day 2: CTRL: 12.4 ± 0.8; cKO: 14.6 ± 0.7, p = 0.048, Unpaired t -test. Day 3: CTRL: 21.2 ± 3.6; cKO: 34.9 ± 4.5, p = 0.03, Mann-Whitney test. CTRL: n = 29 mice, cKO: n = 24 mice). Data are presented as mean ± S.E.M. * denotes p < 0.05, ** denotes p < 0.01, *** denotes p < 0.005, ns = not significant. This figure illustrates that astrocytic UPF2 depletion is associated with avoidance behavior and increased baseline freezing.
Nir Backlit Epm Apparatus Catalog Env 564a Medassociates, supplied by Med Associates Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nir backlit epm apparatus catalog env 564a medassociates - by Bioz Stars, 2026-05
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elevated plus maze apparatus for mice  (Stoelting inc)
90
Stoelting inc elevated plus maze apparatus for mice
( A ) Open field beam breaks display no differences in locomotion amongst CTRL and cKO animals (CTRL: 3892 ± 271; cKO: 4076 ± 239, n = 22 mice per group, p = 0.61, Unpaired t -test). ( B ) Upf2 cKO mice show reduced time in open arms of <t>elevated</t> <t>plus</t> <t>maze</t> indicating anxiety-like behavior (CTRL: 38.5 ± 3.9; cKO: 23.09 ± 2.6, n = 31 mice per group, p = 0.0036, Unpaired t -test). ( C ) Astrocytic Upf2 loss is associated with reduced marble burying (CTRL: 16.3 ± 0.6, n = 22 mice; cKO: 13.0 ± 0.8, n = 23 mice, p = 0.0003, Mann-Whitney test). ( D ) Contextual fear conditioning paradigm revealed differences in contextual memory on Day 2 between CTRL and cKO animals (CTRL: 68.3 ± 3, n = 29 mice; cKO: 77.6 ± 3, n = 24 mice, p = 0.02, Mann-Whitney test). No differences were detected on Day 1 and Day 3 (Day 1: Baseline vs. UC-CS pairings, CTRL vs. cKO, p > 0.59, Sidak’s multiple comparisons test; Day 3: CTRL vs. cKO, p = 0.69, Unpaired t -test). Assessment of baseline freezing revealed Upf2 cKO mice show significant elevation on Day 2 and Day 3 as compared to CTRL (Day 1: CTRL: 1.8 ± 0.6; cKO: 6.1 ± 2.1, p = 0.43, Mann-Whitney test. Day 2: CTRL: 12.4 ± 0.8; cKO: 14.6 ± 0.7, p = 0.048, Unpaired t -test. Day 3: CTRL: 21.2 ± 3.6; cKO: 34.9 ± 4.5, p = 0.03, Mann-Whitney test. CTRL: n = 29 mice, cKO: n = 24 mice). Data are presented as mean ± S.E.M. * denotes p < 0.05, ** denotes p < 0.01, *** denotes p < 0.005, ns = not significant. This figure illustrates that astrocytic UPF2 depletion is associated with avoidance behavior and increased baseline freezing.
Elevated Plus Maze Apparatus For Mice, supplied by Stoelting inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/elevated plus maze apparatus for mice/product/Stoelting inc
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elevated plus maze apparatus for mice - by Bioz Stars, 2026-05
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elevated plus maze apparatus for mice (stoelting co., usa)  (Stoelting inc)
90
Stoelting inc elevated plus maze apparatus for mice (stoelting co., usa)
( A ) Open field beam breaks display no differences in locomotion amongst CTRL and cKO animals (CTRL: 3892 ± 271; cKO: 4076 ± 239, n = 22 mice per group, p = 0.61, Unpaired t -test). ( B ) Upf2 cKO mice show reduced time in open arms of <t>elevated</t> <t>plus</t> <t>maze</t> indicating anxiety-like behavior (CTRL: 38.5 ± 3.9; cKO: 23.09 ± 2.6, n = 31 mice per group, p = 0.0036, Unpaired t -test). ( C ) Astrocytic Upf2 loss is associated with reduced marble burying (CTRL: 16.3 ± 0.6, n = 22 mice; cKO: 13.0 ± 0.8, n = 23 mice, p = 0.0003, Mann-Whitney test). ( D ) Contextual fear conditioning paradigm revealed differences in contextual memory on Day 2 between CTRL and cKO animals (CTRL: 68.3 ± 3, n = 29 mice; cKO: 77.6 ± 3, n = 24 mice, p = 0.02, Mann-Whitney test). No differences were detected on Day 1 and Day 3 (Day 1: Baseline vs. UC-CS pairings, CTRL vs. cKO, p > 0.59, Sidak’s multiple comparisons test; Day 3: CTRL vs. cKO, p = 0.69, Unpaired t -test). Assessment of baseline freezing revealed Upf2 cKO mice show significant elevation on Day 2 and Day 3 as compared to CTRL (Day 1: CTRL: 1.8 ± 0.6; cKO: 6.1 ± 2.1, p = 0.43, Mann-Whitney test. Day 2: CTRL: 12.4 ± 0.8; cKO: 14.6 ± 0.7, p = 0.048, Unpaired t -test. Day 3: CTRL: 21.2 ± 3.6; cKO: 34.9 ± 4.5, p = 0.03, Mann-Whitney test. CTRL: n = 29 mice, cKO: n = 24 mice). Data are presented as mean ± S.E.M. * denotes p < 0.05, ** denotes p < 0.01, *** denotes p < 0.005, ns = not significant. This figure illustrates that astrocytic UPF2 depletion is associated with avoidance behavior and increased baseline freezing.
Elevated Plus Maze Apparatus For Mice (Stoelting Co., Usa), supplied by Stoelting inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/elevated plus maze apparatus for mice (stoelting co., usa)/product/Stoelting inc
Average 90 stars, based on 1 article reviews
elevated plus maze apparatus for mice (stoelting co., usa) - by Bioz Stars, 2026-05
90/100 stars
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elevated-plus maze apparatus  (Panlab)
90
Panlab elevated-plus maze apparatus
( A ) Open field beam breaks display no differences in locomotion amongst CTRL and cKO animals (CTRL: 3892 ± 271; cKO: 4076 ± 239, n = 22 mice per group, p = 0.61, Unpaired t -test). ( B ) Upf2 cKO mice show reduced time in open arms of <t>elevated</t> <t>plus</t> <t>maze</t> indicating anxiety-like behavior (CTRL: 38.5 ± 3.9; cKO: 23.09 ± 2.6, n = 31 mice per group, p = 0.0036, Unpaired t -test). ( C ) Astrocytic Upf2 loss is associated with reduced marble burying (CTRL: 16.3 ± 0.6, n = 22 mice; cKO: 13.0 ± 0.8, n = 23 mice, p = 0.0003, Mann-Whitney test). ( D ) Contextual fear conditioning paradigm revealed differences in contextual memory on Day 2 between CTRL and cKO animals (CTRL: 68.3 ± 3, n = 29 mice; cKO: 77.6 ± 3, n = 24 mice, p = 0.02, Mann-Whitney test). No differences were detected on Day 1 and Day 3 (Day 1: Baseline vs. UC-CS pairings, CTRL vs. cKO, p > 0.59, Sidak’s multiple comparisons test; Day 3: CTRL vs. cKO, p = 0.69, Unpaired t -test). Assessment of baseline freezing revealed Upf2 cKO mice show significant elevation on Day 2 and Day 3 as compared to CTRL (Day 1: CTRL: 1.8 ± 0.6; cKO: 6.1 ± 2.1, p = 0.43, Mann-Whitney test. Day 2: CTRL: 12.4 ± 0.8; cKO: 14.6 ± 0.7, p = 0.048, Unpaired t -test. Day 3: CTRL: 21.2 ± 3.6; cKO: 34.9 ± 4.5, p = 0.03, Mann-Whitney test. CTRL: n = 29 mice, cKO: n = 24 mice). Data are presented as mean ± S.E.M. * denotes p < 0.05, ** denotes p < 0.01, *** denotes p < 0.005, ns = not significant. This figure illustrates that astrocytic UPF2 depletion is associated with avoidance behavior and increased baseline freezing.
Elevated Plus Maze Apparatus, supplied by Panlab, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/elevated-plus maze apparatus/product/Panlab
Average 90 stars, based on 1 article reviews
elevated-plus maze apparatus - by Bioz Stars, 2026-05
90/100 stars
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Image Search Results


( A ) Open field beam breaks display no differences in locomotion amongst CTRL and cKO animals (CTRL: 3892 ± 271; cKO: 4076 ± 239, n = 22 mice per group, p = 0.61, Unpaired t -test). ( B ) Upf2 cKO mice show reduced time in open arms of elevated plus maze indicating anxiety-like behavior (CTRL: 38.5 ± 3.9; cKO: 23.09 ± 2.6, n = 31 mice per group, p = 0.0036, Unpaired t -test). ( C ) Astrocytic Upf2 loss is associated with reduced marble burying (CTRL: 16.3 ± 0.6, n = 22 mice; cKO: 13.0 ± 0.8, n = 23 mice, p = 0.0003, Mann-Whitney test). ( D ) Contextual fear conditioning paradigm revealed differences in contextual memory on Day 2 between CTRL and cKO animals (CTRL: 68.3 ± 3, n = 29 mice; cKO: 77.6 ± 3, n = 24 mice, p = 0.02, Mann-Whitney test). No differences were detected on Day 1 and Day 3 (Day 1: Baseline vs. UC-CS pairings, CTRL vs. cKO, p > 0.59, Sidak’s multiple comparisons test; Day 3: CTRL vs. cKO, p = 0.69, Unpaired t -test). Assessment of baseline freezing revealed Upf2 cKO mice show significant elevation on Day 2 and Day 3 as compared to CTRL (Day 1: CTRL: 1.8 ± 0.6; cKO: 6.1 ± 2.1, p = 0.43, Mann-Whitney test. Day 2: CTRL: 12.4 ± 0.8; cKO: 14.6 ± 0.7, p = 0.048, Unpaired t -test. Day 3: CTRL: 21.2 ± 3.6; cKO: 34.9 ± 4.5, p = 0.03, Mann-Whitney test. CTRL: n = 29 mice, cKO: n = 24 mice). Data are presented as mean ± S.E.M. * denotes p < 0.05, ** denotes p < 0.01, *** denotes p < 0.005, ns = not significant. This figure illustrates that astrocytic UPF2 depletion is associated with avoidance behavior and increased baseline freezing.

Journal: bioRxiv

Article Title: Astrocytic RNA degradation suppresses calcium signaling to support synapse function and restrain anxiety

doi: 10.1101/2025.11.18.689044

Figure Lengend Snippet: ( A ) Open field beam breaks display no differences in locomotion amongst CTRL and cKO animals (CTRL: 3892 ± 271; cKO: 4076 ± 239, n = 22 mice per group, p = 0.61, Unpaired t -test). ( B ) Upf2 cKO mice show reduced time in open arms of elevated plus maze indicating anxiety-like behavior (CTRL: 38.5 ± 3.9; cKO: 23.09 ± 2.6, n = 31 mice per group, p = 0.0036, Unpaired t -test). ( C ) Astrocytic Upf2 loss is associated with reduced marble burying (CTRL: 16.3 ± 0.6, n = 22 mice; cKO: 13.0 ± 0.8, n = 23 mice, p = 0.0003, Mann-Whitney test). ( D ) Contextual fear conditioning paradigm revealed differences in contextual memory on Day 2 between CTRL and cKO animals (CTRL: 68.3 ± 3, n = 29 mice; cKO: 77.6 ± 3, n = 24 mice, p = 0.02, Mann-Whitney test). No differences were detected on Day 1 and Day 3 (Day 1: Baseline vs. UC-CS pairings, CTRL vs. cKO, p > 0.59, Sidak’s multiple comparisons test; Day 3: CTRL vs. cKO, p = 0.69, Unpaired t -test). Assessment of baseline freezing revealed Upf2 cKO mice show significant elevation on Day 2 and Day 3 as compared to CTRL (Day 1: CTRL: 1.8 ± 0.6; cKO: 6.1 ± 2.1, p = 0.43, Mann-Whitney test. Day 2: CTRL: 12.4 ± 0.8; cKO: 14.6 ± 0.7, p = 0.048, Unpaired t -test. Day 3: CTRL: 21.2 ± 3.6; cKO: 34.9 ± 4.5, p = 0.03, Mann-Whitney test. CTRL: n = 29 mice, cKO: n = 24 mice). Data are presented as mean ± S.E.M. * denotes p < 0.05, ** denotes p < 0.01, *** denotes p < 0.005, ns = not significant. This figure illustrates that astrocytic UPF2 depletion is associated with avoidance behavior and increased baseline freezing.

Article Snippet: Elevated Plus Maze : The Elevated plus maze apparatus was positioned and set for Noldus Ethovision XT software acquisition.

Techniques: MANN-WHITNEY

( A ) Scheme for viral delivery and timeline for Ca 2+ measurement experiments. ( B ) CalEx diminished Ca 2+ events to CTRL levels in hippocampus (CTRL: 4.5 ± 1.5, n = 6 mice, 32 ROIs; cKO: 5.1 ± 0.9, n = 8 mice, 68 ROIs; cKO + CalEx: 1.4 ± 0.1, n = 4 mice, 46 ROIs, CTRL vs. cKO + CalEx, p = 0.09, Tukey’s multiple comparisons test). CalEx Ca 2+ events are comparable to CTRL in visual cortex (CTRL: 4.9 ± 1.7, n = 4 mice, 16 ROIs; cKO: 6.6 ± 2.1, n = 6 mice, 36 ROIs; cKO + CalEx: 2.3 ± 0.4, n = 4 mice, 19 ROIs; CTRL vs. cKO + CalEx, p = 0.71, Tukey’s multiple comparisons test). CalEx prefrontal cortex Ca 2+ events are similar to CTRL (CTRL: 0.8 ± 0.2, n = 4 mice, 15 ROIs; cKO: 2.3 ± 0.4, n = 4 mice, 20 ROIs; cKO + CalEx: 1.4 ± 0.3, n = 4 mice, 16 ROIs; CTRL vs. cKO + CalEx, p = 0.54, Tukey’s multiple comparisons test). ( C ) Scheme for viral delivery and tamoxifen treatment followed by rescue assays for PSD-95 density, neurotransmission, and animal behavior. ( D ) Total PSD-95 density is not restored to CTRL levels by CalEx manipulation in Upf2 cKO mice (CTRL: 1648 ± 188, n = 24 ROIs, 4 mice; cKO: 459 ± 41, n = 21 ROIs, 4 mice; cKO + CalEx: 771 ± 52, n = 24 ROIs, 4 mice; CTRL vs. cKO + CalEx, p < 0.0001, Tukey’s multiple comparisons test). ( E ) CalEx elevates synaptic strength in hippocampus of Upf2 cKO mice as compared to CTRL (CTRL: Linear fit 1.88 ± 0.1, r = 0.99, n = 6 slices, 3 mice; cKO: Linear fit 0.84 ± 0.05, r = 0.99, n = 5 slices, 4 mice; cKO + CalEx: Linear fit 2.66 ± 0.24, r = 0.98, n = 5 slices, 3 mice. Linear fit slope values: CTRL vs. cKO + CalEx, p = 0.03, Tukey’s multiple comparisons test). ( F ) CalEx increases elevated plus maze open arm time in Upf2 cKO mice (CTRL: 70.7 ± 5.7, n = 11 mice; cKO: 25.9 ± 5.2, n = 8 mice; cKO + CalEx: 49.1 ± 8.1, n = 10 mice; CTRL + CalEx: 40.6 ± 6.4, n = 8 mice, CTRL vs. cKO + CalEx, p = 0.08, Tukey’s multiple comparisons test). Data are presented as mean ± S.E.M. * denotes p < 0.05, ** denotes p < 0.01, **** denotes p < 0.0001, ns = not significant. This figure depicts that restoring Ca 2+ levels in astrocytes of Upf2 cKO mice recovers basal

Journal: bioRxiv

Article Title: Astrocytic RNA degradation suppresses calcium signaling to support synapse function and restrain anxiety

doi: 10.1101/2025.11.18.689044

Figure Lengend Snippet: ( A ) Scheme for viral delivery and timeline for Ca 2+ measurement experiments. ( B ) CalEx diminished Ca 2+ events to CTRL levels in hippocampus (CTRL: 4.5 ± 1.5, n = 6 mice, 32 ROIs; cKO: 5.1 ± 0.9, n = 8 mice, 68 ROIs; cKO + CalEx: 1.4 ± 0.1, n = 4 mice, 46 ROIs, CTRL vs. cKO + CalEx, p = 0.09, Tukey’s multiple comparisons test). CalEx Ca 2+ events are comparable to CTRL in visual cortex (CTRL: 4.9 ± 1.7, n = 4 mice, 16 ROIs; cKO: 6.6 ± 2.1, n = 6 mice, 36 ROIs; cKO + CalEx: 2.3 ± 0.4, n = 4 mice, 19 ROIs; CTRL vs. cKO + CalEx, p = 0.71, Tukey’s multiple comparisons test). CalEx prefrontal cortex Ca 2+ events are similar to CTRL (CTRL: 0.8 ± 0.2, n = 4 mice, 15 ROIs; cKO: 2.3 ± 0.4, n = 4 mice, 20 ROIs; cKO + CalEx: 1.4 ± 0.3, n = 4 mice, 16 ROIs; CTRL vs. cKO + CalEx, p = 0.54, Tukey’s multiple comparisons test). ( C ) Scheme for viral delivery and tamoxifen treatment followed by rescue assays for PSD-95 density, neurotransmission, and animal behavior. ( D ) Total PSD-95 density is not restored to CTRL levels by CalEx manipulation in Upf2 cKO mice (CTRL: 1648 ± 188, n = 24 ROIs, 4 mice; cKO: 459 ± 41, n = 21 ROIs, 4 mice; cKO + CalEx: 771 ± 52, n = 24 ROIs, 4 mice; CTRL vs. cKO + CalEx, p < 0.0001, Tukey’s multiple comparisons test). ( E ) CalEx elevates synaptic strength in hippocampus of Upf2 cKO mice as compared to CTRL (CTRL: Linear fit 1.88 ± 0.1, r = 0.99, n = 6 slices, 3 mice; cKO: Linear fit 0.84 ± 0.05, r = 0.99, n = 5 slices, 4 mice; cKO + CalEx: Linear fit 2.66 ± 0.24, r = 0.98, n = 5 slices, 3 mice. Linear fit slope values: CTRL vs. cKO + CalEx, p = 0.03, Tukey’s multiple comparisons test). ( F ) CalEx increases elevated plus maze open arm time in Upf2 cKO mice (CTRL: 70.7 ± 5.7, n = 11 mice; cKO: 25.9 ± 5.2, n = 8 mice; cKO + CalEx: 49.1 ± 8.1, n = 10 mice; CTRL + CalEx: 40.6 ± 6.4, n = 8 mice, CTRL vs. cKO + CalEx, p = 0.08, Tukey’s multiple comparisons test). Data are presented as mean ± S.E.M. * denotes p < 0.05, ** denotes p < 0.01, **** denotes p < 0.0001, ns = not significant. This figure depicts that restoring Ca 2+ levels in astrocytes of Upf2 cKO mice recovers basal

Article Snippet: Elevated Plus Maze : The Elevated plus maze apparatus was positioned and set for Noldus Ethovision XT software acquisition.

Techniques: